Bioluminescence Assays: Standard ATP vs. AK Amplified ATP Celsis’ AKuScreen assay with patented AK-amplified ATP bioluminescence generates results in 18-24 hours: 25% - 50% faster than the industry standard ATP bioluminescence. About ATP All living organisms contain the compound ATP (adenosine triphosphate) as a vital part of their energy metabolism. It is this ATP that is released and detected by a standard bioluminescence assay. In the presence of the luciferase enzyme, ATP reacts with luciferin and oxygen to emit a photon of yellow-green light, similar to that of a firefly's tail. The software-controlled luminometer automatically adds the required reagents to a sample and detects the emitted light with great sensitivity. Because ATP is only present in living organisms, Celsis systems are not confused by the presence of dead cells – a common complaint of flow cytometry. ATP bioluminescence technology has been around for 40 years, but only since the introduction of Celsis' selective extraction reagents has the technology been commercialized for use in a broad range of manufacturing industries. ATP and AK Although standard ATP bioluminescence is a very sensitive technique, it is limited by the fact that it is detecting a metabolite (ATP), and an organism can contain only a finite amount of ATP. All living organisms also contain adenylate kinase (AK), another vital part of energy metabolism. Because this is an enzyme, rather than a metabolite, it is possible to use AK to generate almost unlimited amounts of its products. After just 25 minutes, the amount of ATP can be 1000-fold more than the organism originally contained. See the demo (requires Flash) To learn more, download our whitepaper, view additional materials and reports online in our resource center, or contact Celsis Rapid Detection.
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