Amplifying ATP: Standard vs. AK Enhanced Assays Celsis’ proprietary AK-enhanced ATP generates results in 18-24 hours: 25% - 50% faster than standard ATP rapid detection. About ATP All living organisms contain the compound ATP (adenosine triphosphate) as a vital part of their energy metabolism. It is this ATP that is released and detected by a standard bioluminescence test. ATP rapidly reacts, in the presence of the luciferase enzyme, with luciferin and oxygen to give a photon of yellow-green light, similar to that of a firefly. A luminometer automatically adds the required reagents to a sample and detects the emitted light with great sensitivity. Although standard ATP bioluminescence is a very sensitive technique, it is limited by the fact that it is detecting a metabolite (ATP), and an organism can contain only a finite amount of ATP. ATP and AK All living organisms also contain adenylate kinase (AK), another vital part of energy metabolism. Because this is an enzyme, rather than a metabolite, it is possible to use AK to generate almost unlimited amounts of its products. After just 25 minutes, the amount of ATP can be 1000-fold more than the organism originally contained. See the demo (requires Flash) To learn more, download our whitepaper, view additional materials and reports online in our resource center, or contact Celsis Rapid Detection. |
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Rapid Detection |
